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complete intesticult growth medium (mouse) 6005  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc complete intesticult growth medium (mouse) 6005
    Sheep enteroids cultured in an <t>Intesticult</t> medium alone. ( a ) Representative images showing the growth and development of individual enteroids over 9 days of culture. Scale bar, 100 μm. ( b ) Second-generation growth state after passaging (P1: passage 1; D1: day 1 post-passage). Scale bar, 100 μm. ( c ) Growth status of organoids across days in the first three generations. ( d ) Statistical analysis of 9-day area changes in first-generation organoids (ANOVA, p < 0.05). ( e ) Statistical analysis of 7-day germination rates for first-generation organoids (ANOVA, p < 0.01). ( f ) Germination rate comparison among the first three generations (ANOVA, p < 0.05). Scale bar, 100 μm. (Concentrate: d–f: Individual data plots are presented. Data are derived from three individual organoids distributed among three individual wells. These wells are from one different culture batch. A total of one animal was used in the experiment to generate the raw counts shown in the graphs.).
    Complete Intesticult Growth Medium (Mouse) 6005, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complete intesticult growth medium (mouse) 6005/product/STEMCELL Technologies Inc
    Average 90 stars, based on 1 article reviews
    complete intesticult growth medium (mouse) 6005 - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "IL-1β Induced Intestinal Inflammation Pathogenesis in East Friesian Sheep: Insights from Organoid Modeling"

    Article Title: IL-1β Induced Intestinal Inflammation Pathogenesis in East Friesian Sheep: Insights from Organoid Modeling

    Journal: Animals : an Open Access Journal from MDPI

    doi: 10.3390/ani15081097

    Sheep enteroids cultured in an Intesticult medium alone. ( a ) Representative images showing the growth and development of individual enteroids over 9 days of culture. Scale bar, 100 μm. ( b ) Second-generation growth state after passaging (P1: passage 1; D1: day 1 post-passage). Scale bar, 100 μm. ( c ) Growth status of organoids across days in the first three generations. ( d ) Statistical analysis of 9-day area changes in first-generation organoids (ANOVA, p < 0.05). ( e ) Statistical analysis of 7-day germination rates for first-generation organoids (ANOVA, p < 0.01). ( f ) Germination rate comparison among the first three generations (ANOVA, p < 0.05). Scale bar, 100 μm. (Concentrate: d–f: Individual data plots are presented. Data are derived from three individual organoids distributed among three individual wells. These wells are from one different culture batch. A total of one animal was used in the experiment to generate the raw counts shown in the graphs.).
    Figure Legend Snippet: Sheep enteroids cultured in an Intesticult medium alone. ( a ) Representative images showing the growth and development of individual enteroids over 9 days of culture. Scale bar, 100 μm. ( b ) Second-generation growth state after passaging (P1: passage 1; D1: day 1 post-passage). Scale bar, 100 μm. ( c ) Growth status of organoids across days in the first three generations. ( d ) Statistical analysis of 9-day area changes in first-generation organoids (ANOVA, p < 0.05). ( e ) Statistical analysis of 7-day germination rates for first-generation organoids (ANOVA, p < 0.01). ( f ) Germination rate comparison among the first three generations (ANOVA, p < 0.05). Scale bar, 100 μm. (Concentrate: d–f: Individual data plots are presented. Data are derived from three individual organoids distributed among three individual wells. These wells are from one different culture batch. A total of one animal was used in the experiment to generate the raw counts shown in the graphs.).

    Techniques Used: Cell Culture, Passaging, Comparison, Derivative Assay



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    Sheep enteroids cultured in an <t>Intesticult</t> medium alone. ( a ) Representative images showing the growth and development of individual enteroids over 9 days of culture. Scale bar, 100 μm. ( b ) Second-generation growth state after passaging (P1: passage 1; D1: day 1 post-passage). Scale bar, 100 μm. ( c ) Growth status of organoids across days in the first three generations. ( d ) Statistical analysis of 9-day area changes in first-generation organoids (ANOVA, p < 0.05). ( e ) Statistical analysis of 7-day germination rates for first-generation organoids (ANOVA, p < 0.01). ( f ) Germination rate comparison among the first three generations (ANOVA, p < 0.05). Scale bar, 100 μm. (Concentrate: d–f: Individual data plots are presented. Data are derived from three individual organoids distributed among three individual wells. These wells are from one different culture batch. A total of one animal was used in the experiment to generate the raw counts shown in the graphs.).
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    Image Search Results


    Sheep enteroids cultured in an Intesticult medium alone. ( a ) Representative images showing the growth and development of individual enteroids over 9 days of culture. Scale bar, 100 μm. ( b ) Second-generation growth state after passaging (P1: passage 1; D1: day 1 post-passage). Scale bar, 100 μm. ( c ) Growth status of organoids across days in the first three generations. ( d ) Statistical analysis of 9-day area changes in first-generation organoids (ANOVA, p < 0.05). ( e ) Statistical analysis of 7-day germination rates for first-generation organoids (ANOVA, p < 0.01). ( f ) Germination rate comparison among the first three generations (ANOVA, p < 0.05). Scale bar, 100 μm. (Concentrate: d–f: Individual data plots are presented. Data are derived from three individual organoids distributed among three individual wells. These wells are from one different culture batch. A total of one animal was used in the experiment to generate the raw counts shown in the graphs.).

    Journal: Animals : an Open Access Journal from MDPI

    Article Title: IL-1β Induced Intestinal Inflammation Pathogenesis in East Friesian Sheep: Insights from Organoid Modeling

    doi: 10.3390/ani15081097

    Figure Lengend Snippet: Sheep enteroids cultured in an Intesticult medium alone. ( a ) Representative images showing the growth and development of individual enteroids over 9 days of culture. Scale bar, 100 μm. ( b ) Second-generation growth state after passaging (P1: passage 1; D1: day 1 post-passage). Scale bar, 100 μm. ( c ) Growth status of organoids across days in the first three generations. ( d ) Statistical analysis of 9-day area changes in first-generation organoids (ANOVA, p < 0.05). ( e ) Statistical analysis of 7-day germination rates for first-generation organoids (ANOVA, p < 0.01). ( f ) Germination rate comparison among the first three generations (ANOVA, p < 0.05). Scale bar, 100 μm. (Concentrate: d–f: Individual data plots are presented. Data are derived from three individual organoids distributed among three individual wells. These wells are from one different culture batch. A total of one animal was used in the experiment to generate the raw counts shown in the graphs.).

    Article Snippet: Plates were incubated at 37 °C, 5% CO 2 for 30 min to allow the Matrigel to polymerize and then 500 μL of prewarmed complete IntestiCult Growth Medium (mouse) (6005; STEMCELL Technologies, Vancouver, BC, Canada) containing 10 μM Y-27632 (HY-10071; MCE, Shanghai, China), 10 μM LY2157299 (SF7926; Beyotime, Shanghai, China) [ ], 25 μg/mL FGF-basic (Z03230; GenScript, Nanjing, China), 100 ng/mL IGF-1 (Z03688; GenScript, Nanjing, China), Gen-2.7%, and P/S-1% added to each well.

    Techniques: Cell Culture, Passaging, Comparison, Derivative Assay